This thesis aims to quantify the mass transfer of proteins through a membrane and understand which driving forces in the system are responsible for protein–protein separation with ultrafiltration (UF).
Ultrafiltration (UF) is a frequently used method for protein separation. Usually the protein is fully retained by the membrane, while the protein is fully retained by the membrane, while smaller molecules permeate, leading to a concentrated protein solution that is purified from the smaller solutes.
Fractionation of proteins from each other requires transport of proteins through a membrane. Since proteins generally have similar molecular weights, this is not trivial and has not been thoroughly studied before.